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M94A3154.TXT
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1994-10-25
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Document 3154
DOCN M94A3154
TI Role of mycoplasma in SIV infection of rhesus macaques.
DT 9412
AU Bahraoui E; Kovacic R; Canarelli S; Guetard D; Hurtrel B; Blanchard A;
Montagnier L; URA 1455 CNRS, Marseille.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):140 (abstract no. PA0181). Unique
Identifier : AIDSLINE ICA10/94369421
AB OBJECTIVE: Here, using rhesus macaque as a model, we investigated the
susceptibility of these animals to mycoplasma infection and the role of
Mycoplasma penetrans, Mycoplasma pirum and Mycoplasma fermentans in
simian immunodeficiency virus (SIV) replication and pathogenicity.
MATERIALS AND METHODS: Rhesus macaques were used for infection with
mycoplasma alone, SIV alone, both mycoplasma and SIV naive and immunized
animals against various mycoplasma antigens. Blood samples were drawn
every two or three days post infection, and then each 4 weeks, for the
evaluation of SIV P27 antigenemia and PBMC PCR. Specific humoral
responses against SIV and mycoplasma were assessed by Western blot (WB)
and radioimmunoassays (RIA). RESULTS: Here we show, using PCR, WB and
RIA assays, the susceptibility of rhesus macaques to Mycoplasma
fermentans infection, thus making rhesus macaques a valuable model for
the study of the effect of mycoplasma infection on the development of
SIV pathogenicity. All three groups of macaques immunized with M.
penetrans, M. pirum and M. fermentans antigens produced high titers of
specific antibodies. These three groups of animals are now challenged
with 10 MID50 (monkey infectious doses) of SIVmac251 and will be
regularly assessed, in comparison with non-immunized macaques, by PBMC
coculture, PBMC PCR for SIV and mycoplasma detection, plasma SIV P27,
antibody levels and clinical signs. CONCLUSION: Although this study is
too early to make definitive conclusions, the comparison of virological
parameters and the kinetics of the evolution of this disease in the
macaques immunized or not against mycoplasma antigens will assess the
potential therapeutic benefit of this vaccination.
DE Animal Antibodies, Viral/BLOOD Antigens, Viral/BLOOD Blotting,
Western Lymphocytes/MICROBIOLOGY Macaca mulatta *Mycoplasma
*Mycoplasma fermentans Mycoplasma Infections/*COMPLICATIONS Polymerase
Chain Reaction Radioimmunoassay Simian Acquired Immunodeficiency
Syndrome/*COMPLICATIONS SIV/ISOLATION &
PURIF/*PHYSIOLOGY/*PATHOGENICITY *Virus Replication MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).